If you decided not to hire a car, you can go from Rome to Siena by bus (2h45min, 15-24€) or train (3-4h, 16-37€). We recomend the bus as it is direct (by train you need to do some transfers), it takes more or less the same time, has a similar cost and arrives in the historic center of Siena (the train leaves you a little outside).
Baltour group buses (Baltour, ...
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If you have no rental car, go towards Piazza Gramsci station and buy a ticket to San Gimignano. It is bus 130 (65min, 6 €) and it will leave at the main city gate (Porta San Giovanni). Find the hours at this schedule. Take into account that some buses stop at Poggibonsi, where you have to change to another bus to reach San Gimignano. They will indicate you if that is ...
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If you have no rental car, you can go from San Gimignano to Florence via Poggibonsi. You have two alternatives: bus + train combination or bus + bus combination, being the latter most convenient. Go to the tourist information office and buy the bus ticket from San Gimignano to Florence (1h30min, 7€). Bus 130 leaves from Porta San Giovanni and will take you to ...
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